Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.
Identifieur interne : 000906 ( Main/Exploration ); précédent : 000905; suivant : 000907Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.
Auteurs : Yunxing Zhang [République populaire de Chine] ; Xiaojiao Han [République populaire de Chine] ; Shuangshuang Chen [République populaire de Chine] ; Liu Zheng [République populaire de Chine] ; Xuelian He [République populaire de Chine] ; Mingying Liu [République populaire de Chine] ; Guirong Qiao [République populaire de Chine] ; Yang Wang [République populaire de Chine] ; Renying Zhuo [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2017.
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Abstract
Salix matsudana is a deciduous, rapidly growing willow species commonly cultivated in China, which can tolerate drought, salt, and heavy metal stress conditions. Selection of suitable reference genes for quantitative real-time PCR is important for normalizing the expression of the key genes associated with various stresses. To validate suitable reference genes, we selected 11 candidate reference genes (five traditional housekeeping genes and six novel genes) and analyzed their expression stability in various samples, including different tissues and under different abiotic stress treatments. The expression of these genes was determined using five programs-geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder. The results showed that α-TUB2 (alpha-tubulin 2) and DnaJ (chaperone protein DnaJ 49) were the most stable reference genes across all the tested samples. We measured the expression profiles of the defense response gene SmCAT (catalase) using the two most stable and one least stable reference genes in all samples of S. matsudana. The relative quantification of SmCAT varied greatly according to the different reference genes. We propose that α-TUB2 and DnaJ should be the preferred reference genes for normalization and quantification of transcript levels in future gene expression studies in willow species under various abiotic stress conditions.
DOI: 10.1038/srep40290
PubMed: 28120870
PubMed Central: PMC5264508
Affiliations:
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Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28120870</idno><idno type="pmid">28120870</idno><idno type="doi">10.1038/srep40290</idno><idno type="pmc">PMC5264508</idno><idno type="wicri:Area/Main/Corpus">000A48</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A48</idno><idno type="wicri:Area/Main/Curation">000A48</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A48</idno><idno type="wicri:Area/Main/Exploration">000A48</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.</title><author><name sortKey="Zhang, Yunxing" sort="Zhang, Yunxing" uniqKey="Zhang Y" first="Yunxing" last="Zhang">Yunxing Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo, Henan 454000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo, Henan 454000</wicri:regionArea><wicri:noRegion>Henan 454000</wicri:noRegion></affiliation></author><author><name sortKey="Han, Xiaojiao" sort="Han, Xiaojiao" uniqKey="Han X" first="Xiaojiao" last="Han">Xiaojiao Han</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Shuangshuang" sort="Chen, Shuangshuang" uniqKey="Chen S" first="Shuangshuang" last="Chen">Shuangshuang Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Liu" sort="Zheng, Liu" uniqKey="Zheng L" first="Liu" last="Zheng">Liu Zheng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="He, Xuelian" sort="He, Xuelian" uniqKey="He X" first="Xuelian" last="He">Xuelian He</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Mingying" sort="Liu, Mingying" uniqKey="Liu M" first="Mingying" last="Liu">Mingying Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="Qiao, Guirong" sort="Qiao, Guirong" uniqKey="Qiao G" first="Guirong" last="Qiao">Guirong Qiao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yang" sort="Wang, Yang" uniqKey="Wang Y" first="Yang" last="Wang">Yang Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201</wicri:regionArea><wicri:noRegion>Yunnan 650201</wicri:noRegion></affiliation></author><author><name sortKey="Zhuo, Renying" sort="Zhuo, Renying" uniqKey="Zhuo R" first="Renying" last="Zhuo">Renying Zhuo</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400</wicri:regionArea><wicri:noRegion>Zhejiang 311400</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algorithms (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reference Standards (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Salix (genetics)</term><term>Stress, Physiological (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algorithmes (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Normes de référence (MeSH)</term><term>Reproductibilité des résultats (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (méthodes)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Salix (génétique)</term><term>Stress physiologique (génétique)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Salix</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Salix</term><term>Stress physiologique</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaine en temps réel</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Algorithms</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Reference Standards</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algorithmes</term><term>Gènes de plante</term><term>Normes de référence</term><term>Reproductibilité des résultats</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Salix matsudana is a deciduous, rapidly growing willow species commonly cultivated in China, which can tolerate drought, salt, and heavy metal stress conditions. Selection of suitable reference genes for quantitative real-time PCR is important for normalizing the expression of the key genes associated with various stresses. To validate suitable reference genes, we selected 11 candidate reference genes (five traditional housekeeping genes and six novel genes) and analyzed their expression stability in various samples, including different tissues and under different abiotic stress treatments. The expression of these genes was determined using five programs-geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder. The results showed that α-TUB2 (alpha-tubulin 2) and DnaJ (chaperone protein DnaJ 49) were the most stable reference genes across all the tested samples. We measured the expression profiles of the defense response gene SmCAT (catalase) using the two most stable and one least stable reference genes in all samples of S. matsudana. The relative quantification of SmCAT varied greatly according to the different reference genes. We propose that α-TUB2 and DnaJ should be the preferred reference genes for normalization and quantification of transcript levels in future gene expression studies in willow species under various abiotic stress conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28120870</PMID><DateCompleted><Year>2018</Year><Month>10</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><PubDate><Year>2017</Year><Month>01</Month><Day>25</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.</ArticleTitle><Pagination><MedlinePgn>40290</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep40290</ELocationID><Abstract><AbstractText>Salix matsudana is a deciduous, rapidly growing willow species commonly cultivated in China, which can tolerate drought, salt, and heavy metal stress conditions. Selection of suitable reference genes for quantitative real-time PCR is important for normalizing the expression of the key genes associated with various stresses. To validate suitable reference genes, we selected 11 candidate reference genes (five traditional housekeeping genes and six novel genes) and analyzed their expression stability in various samples, including different tissues and under different abiotic stress treatments. The expression of these genes was determined using five programs-geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder. The results showed that α-TUB2 (alpha-tubulin 2) and DnaJ (chaperone protein DnaJ 49) were the most stable reference genes across all the tested samples. We measured the expression profiles of the defense response gene SmCAT (catalase) using the two most stable and one least stable reference genes in all samples of S. matsudana. The relative quantification of SmCAT varied greatly according to the different reference genes. We propose that α-TUB2 and DnaJ should be the preferred reference genes for normalization and quantification of transcript levels in future gene expression studies in willow species under various abiotic stress conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yunxing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo, Henan 454000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Xiaojiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shuangshuang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Liu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Xuelian</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Mingying</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiao</LastName><ForeName>Guirong</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhuo</LastName><ForeName>Renying</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000465" MajorTopicYN="N">Algorithms</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012015" MajorTopicYN="N">Reference Standards</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>08</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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first="Shuangshuang" last="Chen">Shuangshuang Chen</name><name sortKey="Chen, Shuangshuang" sort="Chen, Shuangshuang" uniqKey="Chen S" first="Shuangshuang" last="Chen">Shuangshuang Chen</name><name sortKey="Han, Xiaojiao" sort="Han, Xiaojiao" uniqKey="Han X" first="Xiaojiao" last="Han">Xiaojiao Han</name><name sortKey="Han, Xiaojiao" sort="Han, Xiaojiao" uniqKey="Han X" first="Xiaojiao" last="Han">Xiaojiao Han</name><name sortKey="He, Xuelian" sort="He, Xuelian" uniqKey="He X" first="Xuelian" last="He">Xuelian He</name><name sortKey="He, Xuelian" sort="He, Xuelian" uniqKey="He X" first="Xuelian" last="He">Xuelian He</name><name sortKey="Liu, Mingying" sort="Liu, Mingying" uniqKey="Liu M" first="Mingying" last="Liu">Mingying Liu</name><name sortKey="Liu, Mingying" sort="Liu, Mingying" uniqKey="Liu M" first="Mingying" last="Liu">Mingying Liu</name><name sortKey="Qiao, Guirong" sort="Qiao, Guirong" uniqKey="Qiao G" first="Guirong" last="Qiao">Guirong Qiao</name><name sortKey="Qiao, Guirong" sort="Qiao, Guirong" uniqKey="Qiao G" first="Guirong" last="Qiao">Guirong Qiao</name><name sortKey="Wang, Yang" sort="Wang, Yang" uniqKey="Wang Y" first="Yang" last="Wang">Yang Wang</name><name sortKey="Zhang, Yunxing" sort="Zhang, Yunxing" uniqKey="Zhang Y" first="Yunxing" last="Zhang">Yunxing Zhang</name><name sortKey="Zhang, Yunxing" sort="Zhang, Yunxing" uniqKey="Zhang Y" first="Yunxing" last="Zhang">Yunxing Zhang</name><name sortKey="Zheng, Liu" sort="Zheng, Liu" uniqKey="Zheng L" first="Liu" last="Zheng">Liu Zheng</name><name sortKey="Zheng, Liu" sort="Zheng, Liu" uniqKey="Zheng L" first="Liu" last="Zheng">Liu Zheng</name><name sortKey="Zhuo, Renying" sort="Zhuo, Renying" uniqKey="Zhuo R" first="Renying" last="Zhuo">Renying Zhuo</name><name sortKey="Zhuo, Renying" sort="Zhuo, Renying" uniqKey="Zhuo R" first="Renying" last="Zhuo">Renying Zhuo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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